BeNa Culture Collection
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| Culture medium | BNCC352256 |
| Description | Czapek Dox Agar |
| Composition | NaNO3:3.0g, K2HPO4:1.0g, MgSO4:0.5g, KCl:0.5g, FeSO4:0.01g, Sucrose:30.0g, Agar:15.0g |
| Growth conditions | 28 ℃; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 tablets mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | It grows well on Czapek agar, with white, velvety mycelium at the margin and a bluish-green center that turns yellow in later stages. The reverse side of the colony is pale yellow. |
| Sharing mode | Public welfare sharing |
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