BeNa Culture Collection
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| Culture medium | BNCC352255 |
| Description | Streptomyces medium 2 |
| Composition | Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 28 ℃, 24-48 hours, aerobic |
| Subculture procedure | ① Prepare 1-2 plates of the aforementioned fresh medium.② Open the lyophilized vial inside a biosafety cabinet; flame the top of the vial with an alcohol lamp, then promptly apply sterile water to crack it open, followed by breaking the vial with forceps.③ Aspirate 0.5 mL of liquid medium into the lyophilized vial to fully dissolve the contents, then transfer the solution back into the liquid culture tube and mix thoroughly.④ Draw 0.2 mL of the bacterial suspension and inoculate it onto the Agar plate, spreading evenly. Repeat this step to prepare two plates.⑤ Incubate the plates under the aforementioned culture conditions until microbial growth is observed, after which the culture is ready for use. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 2-4mm, circular, with irregular edges, opaque, white on the front, wrinkled on the surface, hard in texture, producing gray white spores, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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