BeNa Culture Collection
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| Culture medium | BNCC352243 |
| Description | LB nutrient agar |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 37 ℃, 18-24h, aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony has a diameter of 2-4mm, irregular shape, irregular edges, opaque, light reddish brown on the front, raised in the middle, rough surface, wrinkled surface, dry texture, does not produce pigments, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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