Fusobacterium necrophorum subspecies|336998 |BNCC

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Fusobacterium necrophorum subsp.necrophorum

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic
Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	Size: 2-4mm Shape: Circular Edge: Neat Transparency: Opaque Color: Grey Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Moist and easy to pick up
Sharing mode	Public welfare sharing