Enteral invasive Escherichia coli|186731 |BNCC

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Escherichia coli EIEC

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare the above 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take about 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving the bacterial powder, pour it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing