Sclerotium griseum|118626 |BNCC

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Aureobasidium pullulans (de Bary) G. Arnaud

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	25-28°C; 3-5 days; aerobic
Subculture procedure	① Prepare 1 test tube containing 5-10 mL liquid medium or sterile water and 2 agar plates; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it apart with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Slowly spreading growth, circular, irregular edges, opaque, gray on the front, dark yellow on the back, flat morphology, dry texture
Sharing mode	Public welfare sharing