Fusobacterium nucleatum subspecies|337608 |BNCC

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Fusobacterium nucleatum subsp. nucleatum

Culture medium	BNCC353538
Description	Liquid thioglycolate culture medium
Composition	Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C₂H₃NaO₂S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Prepare one 18mm test tube of liquid medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing