BeNa Culture Collection
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| Culture medium | BNCC363766 |
| Description | Modified GAM Agar |
| Composition | Proteose Peptone:15.0g, Pancreatic Digest of Casein:10.0g, Soy Peptone:3.0g, Yeast Extract Powder:5.0g, Beef Powder:2.0g, Beef Liver Powder:1.2g, Soluble Starch:0.3g, L-Cysteine:0.15g, Glucose:3.0g, KH2PO4:2.5g, Digested Serum Powder:13.5g, C2H3NaO2S:0.15g, NaCl:3.0g, Sterile 0.1% Vitamin K1 Solution:1mL, Hemin:2.5g, Defibrinated Rabbit Blood:70mL, Kanamycin:100mg, Neomycin:100mg, Vancomycin:1mg, Agar:13.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G(+), Bacillus subtilis |
| Sharing mode | Public welfare sharing |
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