Bacillus subtilis|342457 |BNCC

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Parabacteroides goldsteinii

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 3-4 days; anaerobic;
Subculture procedure	① Prepare 1-2 fresh agar plates as specified above (pre-conditioned in an anaerobic environment for 24 h to remove oxygen).② Working in a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner. Immediately add sterile water to break the seal, then crush the vial using sterile forceps.③ Aspirate 0.5 mL of liquid medium or sterile water (pre-conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly, and repeat to inoculate a second Agar plate.⑤ Incubate the plates under the designated culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 1-2 mm，Shape: Circular，Margin: Irregular，Transparency: Opaque，Color: Grayish-white，Elevation: Raised center，Surface: Glossy and smooth，Texture: Moist and easy to lift，The colonies exhibit an irregular margin with a grayish-white surface that appears darker in color. They are smooth, glossy, and moist in texture, and produce no pigment. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing