BeNa Culture Collection
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| Culture medium | BNCC393078 |
| Description | Middlebrook 7H9 Agar Plate with 25μg/mL Kanamycin (Ready-to-use) |
| Composition | (NH4)2SO4:0.5g, KH2PO4:1.0g, Na2HPO4:2.5g, Na3C6H5O7:0.1g, MgSO4:0.05g, CaCl2:0.0005g, ZnSO4:0.001g, CuSO4:0.001g, L-Glutamic Acid(C5H9NO4):0.5g, C6H8FeNO7:0.04g, Vitamin B6(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.0005g, ADC Supplement:10%(added at 50℃), Agar:18.0g, pH:6.6±0.2(25℃) |
| Growth conditions | 37 ℃; 2-3 weeks; aerobic; |
| Subculture procedure | ① Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. During the cultivation process, the agar plate needs to be kept moist, and 7H10 broth can be added. |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | Green fluorescence |
| Sharing mode | Public welfare sharing |
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