BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Suck 1-3 ml of sterile water (placed in an anaerobic environment for 24 hours) and pour it into a Agar plate. Use a sterile coating rod to scrape the bacterial moss back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface grayish-white, centrally convex, smooth, glossy, moist texture, easily lifted; G+ (blue-violet), bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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