BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30°C, 24-48h, aerobic |
| Subculture procedure | ① Prepare 1-2 YM plates; ② Disinfect the surface of the ampoule. Open it in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it. Crush it with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial. After complete dissolution, directly pipette the suspension (200μl/vial) onto YM plates and spread evenly; ④ Incubate plates under the specified conditions for 24–48 hours. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1–2 mm; circular shape with regular margins; opaque, creamy-white upper surface; centrally convex; glossy surface; moist texture; Gram-positive (blue-violet); oval shape |
| Sharing mode | Public welfare sharing |
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