BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C, 18-24h, aerobic |
| Subculture procedure | ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5mL liquid medium into the lyophilized tube. After complete dissolution, return the solution to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions. The culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; flat morphology; smooth, shiny surface; moist texture; easily liftable; G- (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
UACC-812(UACC-812)
BNCC338200
H22(H22)
BNCC338327
HCC-78(HCC-78)
BNCC338064
NCI-H1373(NCI-H1373)
BNCC341744
HepAD38(HepAD38)
BNCC341745
NCI-H1693(NCI-H1693)
BNCC342281