Curling Lactobacillus|186473 |BNCC

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Lactobacillus crispatus

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1-2mm, circular shape, irregular edge, opaque, pale yellow front, convex (centrally raised), smooth and glossy surface, moist texture, easily picked up, Gram-positive (blue-violet), rod-shaped, purity: pure.
Sharing mode	Public welfare sharing