BeNa Culture Collection
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| Culture medium | BNCC352254 |
| Description | BBL agar medium |
| Composition | Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃) |
| Growth conditions | 37°C; 3-5 days; anaerobic; |
| Subculture procedure | ① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water (pre-equilibrated in anaerobic conditions for 24h) into freeze-dried vial. Thoroughly dissolve bacterial powder, then inoculate 2 plates (200μL/Agar plate) with uniform spread; ④ Incubate plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular with irregular margins; opaque; pale yellow upper surface; central elevation; rough, dull surface; dry texture; Gram-positive (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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