Bifidobacterium adolescentis|186535 |BNCC

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Bifidobacterium adolenscentis

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37°C; 3-5 days; anaerobic;
Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water (pre-equilibrated in anaerobic conditions for 24h) into freeze-dried vial. Thoroughly dissolve bacterial powder, then inoculate 2 plates (200μL/Agar plate) with uniform spread; ④ Incubate plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular with irregular margins; opaque; pale yellow upper surface; central elevation; rough, dull surface; dry texture; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing