BeNa Culture Collection
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| Culture medium | BNCC352238 |
| Description | MRS agar medium |
| Composition | Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37°C, 24-48h, microaerophilic |
| Subculture procedure | ① Prepare 1-2 plates as above (pre-deoxygenate in anaerobic environment for 24h); ② Open in biosafety cabinet, cauterize top with alcohol lamp, then rapidly break with sterile water; crush with forceps; ③ Pipette 0.5mL sterile water into freeze-dried vial, dissolve powder completely, then dispense 200μL/Agar plate, spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, opaque; upper surface milky white with central elevation; smooth texture; moist; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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