Trichoderma viride|339869 |BNCC

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Trichoderma virens

Culture medium	BNCC352256
Description	Czapek Dox Agar
Composition	NaNO₃:3.0g, K₂HPO₄:1.0g, MgSO₄:0.5g, KCl:0.5g, FeSO₄:0.01g, Sucrose:30.0g, Agar:15.0g
Growth conditions	28 ℃; 5-7 days; aerobic
Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow on Chatelier medium, the mycelium is white fuzzy in the early stage and gradually turns yellow. In the later stage, yellow green spores are produced, and the back of the medium is light yellow
Sharing mode	Public welfare sharing