BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 28°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into the freeze-dried vial. After complete dissolution, return it to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Pink Elevation: Central bulge Surface: Bright and smooth Texture: Moist and viscous, G+ (blue-violet), cocci, Purity: Pure |
| Sharing mode | Public welfare sharing |
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