Bifidobacterium rubrum|367933 |BNCC

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Bifidobacterium erythrocebi

Culture medium	BNCC360798
Description	Colombia Agar Chocolate Plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃)
Growth conditions	37°C; 24-48h; anaerobic
Subculture procedure	① Prepare 1-2 plates as above (pre-incubate in anaerobic environment for 24h to remove oxygen); ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water (pre-deoxygenated in anaerobic conditions for 24h) into the freeze-dried vial. After fully dissolving the powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once colonies appear.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet), bacilli; purity: pure
Sharing mode	Public welfare sharing