BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the strain is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, yellowish surface; pale color; central elevation; smooth, glossy surface; moist texture; easily liftable; G− (red); bacillus |
| Sharing mode | Public welfare sharing |
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