Salt dwelling nitrogen fixing bacteria|185361 |BNCC

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Azotobacter salinestris

Culture medium	BNCC353644
Description	Rhizobium Medium 1
Composition	K₂HPO₄:0.5g, MgSO₄.7H₂O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions	28 ℃; 48-72h; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, pink on the front and back, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), cocci, purity: pure
Sharing mode	Public welfare sharing