BeNa Culture Collection
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| Culture medium | BNCC352253 |
| Description | Silicate Bacteria Medium |
| Composition | Glucose:5.0g, Na2HPO4:2.0g, MgSO4:0.5g, FeCl3:0.005g, CaCO3:0.1g, Glass Powder:1.0g, pH:7.0±0.1(25℃) |
| Growth conditions | 28°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 4-6 mm; circular with neat margins; translucent; pale gray upper surface; centrally convex; glossy surface; smooth texture; moist consistency; viscous; Gram-positive (blue-violet); bacilli; purity: pure |
| Sharing mode | Public welfare sharing |
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