Gelatinous Bacillus|193572 |BNCC

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Paenibacillus mucilaginosus

Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	28°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 4-6 mm; circular with neat margins; translucent; pale gray upper surface; centrally convex; glossy surface; smooth texture; moist consistency; viscous; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing

Bacillus mucilaginosus

BNCC No.：193572

Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Culture medium name: silicate bacteria culture medium

2. Medium composition: sucrose 5.0g, disodium hydrogen phosphate 2.0g, magnesium sulfate 0.5g, ferric chloride 0.005g, calcium carbonate 0.1g, bauxite 0.5g,pH7.1±0.2. 121 ℃,15min.

3. Atmosphere：aerobic

4. Temperature：25-28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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