Intermediate Prevotella|352061 |BNCC

Essential Information Certificate Related Products

Prevotella intermedia

Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to the anaerobic workstation for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. ⑤ The anaerobic cultivation environment can also be carried out in a 350ml anaerobic cultivation bag containing anaerobic gas generator.
Growth conditions	37 ℃; 48-72 hours; strictly anaerobic
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, G - (red), short rod-shaped or club shaped, purity: pure
Sharing mode	Public welfare sharing

Prevotella intermedia

Storage conditions: 2~8 ℃

No. 352061

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level:2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:37 ℃, strictly anaerobic, Colombian blood plate (ready-to-use type,3-4 days.

Recovery steps:

(1)Prepare 2 of above mentioned plates(keep in an anaerobic environment for 24 hours);

(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water(Keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 3-4 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 4 days strain layer is obvious and colony is typical
colony morphology: (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: white bulge: middle convex surface: bright and smooth texture: moist and easy to stir
Conclusion	good viability, no abnormal colony morphology, qualified

Download certificate