BeNa Culture Collection
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| Culture medium | BNCC359963 |
| Description | Trypticase Soy Blood Agar |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 30 ° C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony has a diameter of 1–2 mm, is circular with neat edges, and appears opaque. The front side is grayish-white, centrally raised, with a smooth, shiny, and moist surface. It is easily picked up. Gram-negative (red), rod-shaped bacteria. Purity: pure. |
| Sharing mode | Public welfare sharing |
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