Lactobacillus subspecies in mare's milk wine|354841 |BNCC

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Lactobacillus subspecies in mare's milk wine-BNCC
  • BNCC
  • Lactobacillus kefiranofaciens subsp-BNCC

Lactobacillus kefiranofaciens subsp

  • Price： $ 258
  • number：BNCC354841
  • MPs：
    The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a creamy-white pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
  • Packing：Freeze dried pellet; Bacterial suspension; Agar plate
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Lactobacillus kefiranofaciens subsp
Culture medium BNCC352238
Description MRS agar medium
Composition Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions 30 ° C; 5-7 days; anaerobic
Subculture procedure ① Prepare 1-2 plates as described above (pre-conditioned in an anaerobic environment for 24 h for oxygen removal).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water (pre-conditioned in an anaerobic environment for 24 h for oxygen removal) into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions 2-8 ℃
Safety level 1
morphology The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a creamy-white pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
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