Escherichia coli|358230 |BNCC

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Escherichia coli

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30-37 ° C; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 2-4mm Shape: Circular Edge: Irregular Transparency: Opaque; Color: white, raised degree: middle raised, surface: bright and smooth, texture: moist and easy to pick up
Sharing mode	Public welfare sharing

Escherichia coli

Storage conditions : 2~8 ℃

No. : 358230

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30℃, aerobic, blood plate medium (TSA + 5% defibrinated sheep blood), 18-24h.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 18-24hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability :	good viability， in 24hours strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 2-4mm shape: round edge: irregular transparency: opaque color: white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion:	good resurrection, no abnormal colony morphology, qualified

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