K599 Agrobacterium tumefaciens|357912 |BNCC

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Agrobacterium rhizogenes K599

Culture medium	BNCC360873
Description	LB Medium with 50μg/mL Streptomycin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	28 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 1-2 plates as described above.② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing