HB101 Escherichia coli|357962 |BNCC

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Escherichia coli HB101

Culture medium	BNCC360873
Description	LB Medium with 50μg/mL Streptomycin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare 2 plates as described above.② Disinfect the surface of the ampoule, then open it in a biosafety cabinet. Briefly flame the top with an alcohol burner, immediately add sterile water to break the seal, and crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a pale yellow pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture. No pigment is produced. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing