Enterococcus faecalis|358278 |BNCC

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Enterococcus faecium

Culture medium	BNCC359963
Description	Trypticase Soy Blood Agar
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	37 ° C; 18-24h; 5%CO2；
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They appear white on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet) and spherical (cocci). Purity: pure.
Sharing mode	Public welfare sharing