BeNa Culture Collection
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| Culture medium | BNCC382470 |
| Description | Tryptic Soy Blood Agar with Hemin and Vitamin K₁ (TSA-HK) |
| Composition | Tryptone:15.0g, Phytone:5.0g, NaCl:5.0g, Sterile defibrinated sheep blood:5%, Agar:15.0g, Hemin:5mg, Menadione(Vitamin K3):0.5mg, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 3-7 days; anaerobic; |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, gray white front, raised middle, smooth surface, bright surface, viscous texture, moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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