BeNa Culture Collection
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| Culture medium | BNCC391054 |
| Description | LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, and 20μg/mL Tetracycline (Ready-to-use) |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare two plates as described above.② Disinfect the surface of the ampoule, open it in a biosafety cabinet, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, and transfer 200 μL of the suspension onto each of the two plates, spreading evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Purity: pure. |
| Sharing mode | Public welfare sharing |
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