Escherichia coli K12 J53 (containing RP4 plasmid)|361521 |BNCC

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Escherichia coli K12 J53 (containing RP4 plasmid)-BNCC
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  • Escherichia coli K12 J53（RP4）-BNCC

Escherichia coli K12 J53（RP4）

  • Price： $ 223
  • number：BNCC361521
  • MPs：
    Purity: pure.
  • Packing：Freeze dried pellet
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Escherichia coli K12 J53（RP4）
Culture medium BNCC391054
Description LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, and 20μg/mL Tetracycline (Ready-to-use)
Composition Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0(25℃)
Growth conditions 37 ℃; 18-24h; aerobic
Subculture procedure ① Prepare two plates as described above.② Disinfect the surface of the ampoule, open it in a biosafety cabinet, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, and transfer 200 μL of the suspension onto each of the two plates, spreading evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions 2-8 ℃
Safety level 1
morphology Purity: pure.
Sharing mode Public welfare sharing
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