Escherichia coli K12 J53 (containing RP4 plasmid)|361521 |BNCC

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Escherichia coli K12 J53（RP4）

Culture medium	BNCC391054
Description	LB Agar Plate with 50μg/mL Ampicillin, 50μg/mL Kanamycin, and 20μg/mL Tetracycline (Ready-to-use)
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare two plates as described above.② Disinfect the surface of the ampoule, open it in a biosafety cabinet, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the ampoule with sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, and transfer 200 μL of the suspension onto each of the two plates, spreading evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	Purity: pure.
Sharing mode	Public welfare sharing