BeNa Culture Collection
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| Culture medium | BNCC362657 |
| Description | LMG Medium 2 |
| Composition | KH2PO4:0.45g, Na2HPO4.12H2O:2.39g, Beef Extract:1.0g, Yeast Extract:2.0g, Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.4(25℃) |
| Growth conditions | 28 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, bright and smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus |
| Sharing mode | Public welfare sharing |
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