BeNa Culture Collection
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| Culture medium | BNCC359963 |
| Description | Trypticase Soy Blood Agar |
| Composition | Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic; |
| Subculture procedure | ① Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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