Neisseria gonorrhoeae|373808 |BNCC

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Neisseria gonorrhoeae (Zopf) Trevisan

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48h；5%CO2；
Subculture procedure	① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	2
morphology	Colony diameter: 1–2 mm; round with entire margins; opaque; grayish-white on the obverse; convex center; smooth surface; moist consistency; easily lifted; Gram-negative (red); cocci.
Sharing mode	Public welfare sharing