BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC370433 |
| Description | de Man, Rogosa and Sharpe (MRS) Broth |
| Composition | Peptone:10.0g, Beef extract:8.0g, Yeast powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, (NH4)2HC6H5O7:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 1-2 fresh test tubes as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, thoroughly dissolve and mix well; ④ Transfer 0.2mL of bacterial suspension into a test tube; ⑤ Cultivate under the above conditions, and use if the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Liquid culture is better, G+(blue purple), Bacillus |
| Sharing mode | Public welfare sharing |
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