BeNa Culture Collection
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| Culture medium | BNCC352255 |
| Description | Streptomyces medium 2 |
| Composition | Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 28°C, 48-72h, aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect the Agar plate surface and open it in a safety cabinet; ③ Pipette 1-3 ml sterile water onto the Agar plate; use a sterile spreader to scrape the bacterial layer back and forth across the Agar plate surface to create a bacterial suspension; ④ Pipette the suspension with a Pasteur pipette and dispense onto fresh plates, spreading evenly; ⑤ Incubate plates under the specified conditions. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; irregular shape with jagged edges; opaque, pale yellow surface; flat morphology; rough texture; dry consistency; Gram-positive (blue-violet); bacilli |
| Sharing mode | Public welfare sharing |
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