Shigella dysenteriae|291532 |BNCC

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Shigella dysenteriae (Shiga) Castellani and Chalmers

Culture medium	BNCC369667
Description	Nutrient Broth (NB)
Composition	Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25℃)
Growth conditions	37°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium or sterile water and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5mL liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 2-4 mm, circular with irregular margins, opaque, pale yellow on top, centrally convex, smooth surface, glossy appearance, moist texture, G- (red), bacillus, purity: pure
Sharing mode	Public welfare sharing