BeNa Culture Collection
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| Culture medium | BNCC369667 |
| Description | Nutrient Broth (NB) |
| Composition | Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 37°C, 18-24h, aerobic |
| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium or sterile water and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5mL liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 2-4 mm, circular with irregular margins, opaque, pale yellow on top, centrally convex, smooth surface, glossy appearance, moist texture, G- (red), bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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