BeNa Culture Collection
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| Culture medium | BNCC352238 |
| Description | MRS agar medium |
| Composition | Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37°C; 24-48h; anaerobic |
| Subculture procedure | ① Prepare 1-2 plates as above and sterile water (pre-placed in anaerobic environment for 24h to remove oxygen); ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, with neat, opaque edges and a raised center. Surface: grayish-white, smooth, glossy, moist texture, easily liftable. Gram-positive (blue-violet), bacilli. Purity: Pure |
| Sharing mode | Public welfare sharing |
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