Streptococcus mutans|341166 |BNCC

Essential Information Related Products

Ruminococcus torques Holdeman and Moore

Culture medium	BNCC353538
Description	Liquid thioglycolate culture medium
Composition	Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C₂H₃NaO₂S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃)
Growth conditions	37 ° C; 48-72h; anaerobic (solid culture medium Columbia blood Agar plate)
Subculture procedure	① Dissolve the Frozen vial in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, use a sterile pipette to aspirate the bacterial solution, and transfer it to a new solid or liquid culture medium (for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen), and complete the cultivation as required;
Storage conditions	-80 ℃
Safety level	1
morphology	G+(blue purple), cocci
Sharing mode	Public welfare sharing