Bacteroides solanacearum|353923 |BNCC

Essential Information Related Products

Bacteroides xylanisolvens

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 48-72h; anaerobic;
Subculture procedure	① Prepare 1-2 fresh plates as above (pre-deoxygenated in anaerobic environment for 24h); ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly break it with sterile water; shatter it with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with regular margins; opaque, grayish-white on top; centrally convex; smooth surface; moist texture; Gram-negative (red); bacillus; purity: pure
Sharing mode	Public welfare sharing