BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | ① Thaw the cryovial in a 37 °C water bath and shake vigorously to dissolve the contents.② Wipe the outer surface of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for further operations.③ Unscrew the cap, aspirate the entire dissolved solution, and inoculate it onto 1–2 agar plates (about 200 μL per plate; if culturing anaerobic bacteria, pre‑condition the medium in an anaerobic environment for 24 h to remove oxygen).④ Spread evenly and then transfer the plates to the specified culture conditions for incubation. |
| Storage conditions | -80℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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