BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; anaerobic |
| Subculture procedure | ① Prepare 1-2 fresh plates as above (pre-deoxygenated in anaerobic environment for 24h); ② Open in a biosafety cabinet, cauterize the top with , then promptly add sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) to rupture it. Subsequently break it into pieces using forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the culture is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Spreading growth, grayish-white on the front, smooth surface, moist texture, G+ (blue-violet), bacillus |
| Sharing mode | Public welfare sharing |
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