Rod shaped bacteria in the sediment of Lake Michigan|371842 |BNCC

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Sediminibacterium magnilacihabitans

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	28 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium or sterile water and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, opaque, yellow on the front, darker in color, raised in the middle, smooth on the surface, easy to pick up, G - (red), Bacillus subtilis
Sharing mode	Public welfare sharing