Star shaped Nocardia|369710 |BNCC

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Nocardia asteroides

Culture medium	BNCC362653
Description	GYM Streptomyces Agar
Composition	Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO₃:2.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	30 ℃; 3-4 days; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 0.5-1 mm in diameter, are circular with irregular margins, and are opaque. They appear white on the surface, have a raised center, and are rough and firm in texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing