Escherichia coli|194754 |BNCC

Essential Information Related Products

Enterococcus hirae

Culture medium	BNCC354756
Description	Improved MRS culture medium
Composition	Peptone:10.0g, Beef Extract:10.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₃:2.0g, K₂HPO₄:2.0g, MnSO₄:0.05g, Tween 80:1.0g, L-Cysteine:0.5g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Prepare two of the aforementioned plates (pre-equilibrate in an anaerobic environment for 24 hours). ② Disinfect the surface of the ampoule vial, open it within a biosafety cabinet, flame the top using an alcohol lamp, then promptly drop sterile water onto it to induce cracking, followed by breaking it with forceps. ③ Aspirate 0.5 mL of sterile water (pre-equilibrated in an anaerobic environment for 24 hours), inject it into the lyophilized vial, thoroughly resuspend the bacterial powder, then inoculate the suspension onto two plates (200 µL per Agar plate) and spread evenly. ④ Incubate the plates under the specified culture conditions mentioned above. The strain is ready for use once growth is observed.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing