BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC354756 |
| Description | Improved MRS culture medium |
| Composition | Peptone:10.0g, Beef Extract:10.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)3:2.0g, K2HPO4:2.0g, MnSO4:0.05g, Tween 80:1.0g, L-Cysteine:0.5g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | ① Prepare two of the aforementioned plates (pre-equilibrate in an anaerobic environment for 24 hours). ② Disinfect the surface of the ampoule vial, open it within a biosafety cabinet, flame the top using an alcohol lamp, then promptly drop sterile water onto it to induce cracking, followed by breaking it with forceps. ③ Aspirate 0.5 mL of sterile water (pre-equilibrated in an anaerobic environment for 24 hours), inject it into the lyophilized vial, thoroughly resuspend the bacterial powder, then inoculate the suspension onto two plates (200 µL per Agar plate) and spread evenly. ④ Incubate the plates under the specified culture conditions mentioned above. The strain is ready for use once growth is observed. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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