Bacillus subtilis|195271 |BNCC

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Bacillus mucilaginosus

Culture medium	BNCC360922
Description	Nitrogen-Free Medium
Composition	Mannitol:10.0g, KH₂PO₄:0.2g, MgSO₄.7H₂O:0.2g, NaCl:0.2g, CaSO₄.2H₂O:0.2g, CaCO₃:5.0g, Agar:15.0g, pH:7.0-7.2(25℃)
Growth conditions	30 ℃, 24-48 hours, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony exhibits a diameter of 1–2 mm, with a circular shape and an irregular margin. It appears transparent, featuring a raised center, a smooth surface, and a moist texture. The colony is easily picked up, stains Gram‑positive (blue‑violet), and consists of rod‑shaped cells (bacilli).
Sharing mode	Public welfare sharing