Marsh Rhodopseudomonas|376257 |BNCC

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Rhodopseudomonas palustris

Culture medium	BNCC360853
Description	Arginine Thymine Yeast extract Pyruvate Agar
Composition	NH₄Cl:1.0g, KH₂PO₄:0.5g, MgCl₂:0.5g, CaCl₂:0.1g, NaCl:1.0g, CH₃COONa:2.0g, Sodium Succinate(C₄H₄Na₂O₄):1.0g, Yeast Extract Powder:0.5g, Peptone:0.5g, FeCl₂:1.8mg, CoCl₂:0.25mg, NiCl₂:0.01mg, CuCl₂:0.01mg, MnCl₂:0.07mg, ZnCl₂:0.1mg, H₃BO₃:0.5mg, Na₂SeO₃:0.01mg, Na₂MoO₄:0.01g, Biotin(C₁₀H₁₆N₂O₃S):0.01mg, Nicotinic Acid(C₆H₅NO₂):0.035mg, Calcium Pantothenate(C₉H₁₆NO₅Ca):0.01mg, Thiamine Hydrochloride(C₁₂H₁₇ClN₄OS.HCl):0.03mg, Vitamin B12(C₆₃H₈₈CoN₁₄O₁₄P):0.005mg, Pyridoxamine Hydrochloride(C₈H₁₂N₂O₂.HCl):0.01mg, p-Aminobenzoic Acid(C₇H₇NO₂):0.02mg, Agar:13.0g, pH:6.8(25℃)
Growth conditions	30 ℃; 5-7 days; Light anaerobic;
Subculture procedure	① Dissolve the Frozen vial in a water bath at 30 ℃ and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, red on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing