Rhizobium genus|196223 |BNCC

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Rhizobium sp.

Culture medium	BNCC353644
Description	Rhizobium Medium 1
Composition	K₂HPO₄:0.5g, MgSO₄.7H₂O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Cultivate the agar plate under the above cultivation conditions for 18-24 hours. The colony diameter is 1-2mm, round, with neat edges, opaque, pink on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure, producing mucus
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 1-2 mm in diameter, circular with an entire margin, and opaque. The colony surface is pink, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram-negative (red), rod-shaped (bacilli). The culture is pure and exhibits mucoid production.
Sharing mode	Public welfare sharing