BeNa Culture Collection
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| Culture medium | BNCC353644 |
| Description | Rhizobium Medium 1 |
| Composition | K2HPO4:0.5g, MgSO4.7H2O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 30 ℃; 48-72h; aerobic; |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the agar plate under the above cultivation conditions for cultivation. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, semi transparent, light pink, protruding in the middle, smooth and bright surface, sticky and moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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