Streptococcus pyogenes|237467 |BNCC

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Streptococcus mitis Andrewes and Horder emend. Judicial Commission

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃, 18-24h, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Cultivate the agar plate under the above cultivation conditions for 18-24 hours. The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), cocci
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies are 1–2 mm in diameter, circular with entire margins, and opaque. The colony surface is light yellow, convex, smooth, and moist in texture. They are easily picked up. The cells are Gram‑positive (blue‑violet) and spherical (cocci).
Sharing mode	Public welfare sharing